Review



chip buffer  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc chip buffer
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Chip Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+chromatin+ip+buffers/SimpleChIP+Chromatin+IP+Buffers/bio_rxiv__64898__2026__01__20__700469-85-7-14
    Average 94 stars, based on 25 article reviews
    chip buffer - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation"

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation

    Journal: bioRxiv

    doi: 10.64898/2026.01.20.700469

    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Figure Legend Snippet: (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.

    Techniques Used: Immunostaining, Transfection, Control, Sequencing, Expressing, Knockdown, Western Blot, Chromatin Immunoprecipitation

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq.
    Article Snippet: Funasaki et al., STAR Protocols 6, 103326 March 21, 2025 a 2024 The Author(s).. Published by Angiogenesis begins as endothelial cells migrate, forming a sprouting tip and subsequent growth-rich stalk cells.. Here, we present a protocol for transcriptomic and epigenomic analyses of tip-like cells in cultured endothelial cells.

    Article Title: Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq
    Article Snippet: TruSeq stranded mRNA library prep kit , Illumina , 20020594. .. SimpleChIP chromatin IP buffers , Cell Signaling Technology , #14231. .. SimpleChIP enzymatic cell lysis buffers A & B , Cell Signaling Technology , #14282.

    Article Title: Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq
    Article Snippet: .. The Buffer A and Buffer B, the 10× ChIP Buffer and the 2x ChIP Elution Buffer can be obtained as SimpleChIP Enzymatic Cell Lysis Buffers A & B and SimpleChIP Chromatin IP Buffers, respectively, from Cell Signaling Technology. ..

    Article Title: Heat shock transcription factor 1 facilitates liver cancer progression by driving super‐enhancer‐mediated transcription of MYCN
    Article Snippet: .. SimpleChIP Chromatin IP buffers (Cell Signaling Technology) were used to extract and resuspend the protein–DNA complexes. ..

    Article Title: BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer.
    Article Snippet: .. The resulting cleared cell lysate was diluted with SimpleChIP Chromatin IP Buffers (CST) and incubated with antibodies and Dynabeads® Protein G at 4◦C to perform the immunoprecipitation. ..

    Article Title: A STAT3/integrin axis accelerates pancreatic cancer initiation and progression
    Article Snippet: .. Chromatin sonication was performed with Covaris E220 focused ultrasonicator as described previously and IPs were performed using SimpleChIP ® Chromatin IP Buffers (Cell Signaling Cat #14231) per the manufacturer recommendations. ..

    Article Title: HES1 potentiates high salt stress response as an enhancer of NFAT5-DNA binding
    Article Snippet: .. ChIP-qPCR analysis was performed using the SimpleChIP Chromatin IP Buffers and the SimpleChIP Enzymatic Cell Lysis Buffers A&B (Cell Signaling technology, Cat#14231, #14282) basically following the manufacturer’s protocol. ..

    Article Title: A STAT3/integrin axis accelerates pancreatic cancer initiation and progression
    Article Snippet: ChIP-Grade Protein G Magnetic Beads , Cell Signaling , 9006. .. SimpleChIP ® Chromatin IP Buffers , Cell Signaling , 14231. ..

    Lysis:

    Article Title: Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq.
    Article Snippet: Funasaki et al., STAR Protocols 6, 103326 March 21, 2025 a 2024 The Author(s).. Published by Angiogenesis begins as endothelial cells migrate, forming a sprouting tip and subsequent growth-rich stalk cells.. Here, we present a protocol for transcriptomic and epigenomic analyses of tip-like cells in cultured endothelial cells.

    Article Title: Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq
    Article Snippet: .. The Buffer A and Buffer B, the 10× ChIP Buffer and the 2x ChIP Elution Buffer can be obtained as SimpleChIP Enzymatic Cell Lysis Buffers A & B and SimpleChIP Chromatin IP Buffers, respectively, from Cell Signaling Technology. ..

    Article Title: HES1 potentiates high salt stress response as an enhancer of NFAT5-DNA binding
    Article Snippet: .. ChIP-qPCR analysis was performed using the SimpleChIP Chromatin IP Buffers and the SimpleChIP Enzymatic Cell Lysis Buffers A&B (Cell Signaling technology, Cat#14231, #14282) basically following the manufacturer’s protocol. ..

    Incubation:

    Article Title: BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer.
    Article Snippet: .. The resulting cleared cell lysate was diluted with SimpleChIP Chromatin IP Buffers (CST) and incubated with antibodies and Dynabeads® Protein G at 4◦C to perform the immunoprecipitation. ..

    Immunoprecipitation:

    Article Title: BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer.
    Article Snippet: .. The resulting cleared cell lysate was diluted with SimpleChIP Chromatin IP Buffers (CST) and incubated with antibodies and Dynabeads® Protein G at 4◦C to perform the immunoprecipitation. ..

    Sonication:

    Article Title: A STAT3/integrin axis accelerates pancreatic cancer initiation and progression
    Article Snippet: .. Chromatin sonication was performed with Covaris E220 focused ultrasonicator as described previously and IPs were performed using SimpleChIP ® Chromatin IP Buffers (Cell Signaling Cat #14231) per the manufacturer recommendations. ..

    ChIP-qPCR:

    Article Title: HES1 potentiates high salt stress response as an enhancer of NFAT5-DNA binding
    Article Snippet: .. ChIP-qPCR analysis was performed using the SimpleChIP Chromatin IP Buffers and the SimpleChIP Enzymatic Cell Lysis Buffers A&B (Cell Signaling technology, Cat#14231, #14282) basically following the manufacturer’s protocol. ..



    Similar Products

    94
    Cell Signaling Technology Inc chip buffer
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Chip Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+chromatin+ip+buffers/SimpleChIP+Chromatin+IP+Buffers/bio_rxiv__64898__2026__01__20__700469-85-7-14
    Average 94 stars, based on 1 article reviews
    chip buffer - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc salt buffer
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Salt Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+chromatin+ip+buffers/SimpleChIP+Chromatin+IP+Buffers/pmc12333503-305-12-24
    Average 94 stars, based on 1 article reviews
    salt buffer - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc simplechip chromatin ip buffers
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Simplechip Chromatin Ip Buffers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+chromatin+ip+buffers/SimpleChIP+Chromatin+IP+Buffers/pmc12445075-396-17-22
    Average 94 stars, based on 1 article reviews
    simplechip chromatin ip buffers - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc chip elution buffer
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Chip Elution Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+chromatin+ip+buffers/SimpleChIP+Enzymatic+Chromatin+IP+Kit/pm40263291-440-12-15
    Average 99 stars, based on 1 article reviews
    chip elution buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc chip seq dna library
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Chip Seq Dna Library, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+chromatin+ip+buffers/SimpleChIP+Chromatin+IP+Buffers/pm39799578-186-30-42
    Average 94 stars, based on 1 article reviews
    chip seq dna library - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.

    Journal: bioRxiv

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation

    doi: 10.64898/2026.01.20.700469

    Figure Lengend Snippet: (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.

    Article Snippet: Nuclei were pelleted, resuspended in 400 μL ChIP buffer (SimpleChIP ® Chromatin IP Buffers, Cell Signaling Technology) supplemented with protease inhibitors, sonicated for 10 min (30 s on/30 s off, medium intensity) using a Bioruptor UCD-200 (Diagenode), and centrifuged at 21,000 × g for 5 min. Fragmented chromatin (200-500 bp) was confirmed by agarose gel electrophoresis.

    Techniques: Immunostaining, Transfection, Control, Sequencing, Expressing, Knockdown, Western Blot, Chromatin Immunoprecipitation